Abstract
T lymphocytes are key players in immunity. Anti-CD3 antibodies activate T cells and promote a suppressive phenotype in vivo. Although the pharmacological use of these antibodies is widely studied, the underlying mechanisms are still poorly understood. Here we describe the response of the non-coding RNA transcriptome of T cells after in vitro stimulation of peripheral blood mononuclear cells (PBMC) by anti-CD3 and demonstrate that several novel long non-coding RNA are associated with antibody treatment. Regulated long intergenic non-coding RNAs were associated with lymphocyte activation and signaling pathways. In particular the lncRNA transcripts WFDC21P and GAPLINC are regulated in stimulated T cell.
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Acknowledgments
We are thankful to CAPES and CNPq for scholarship funding and to FAPDF for financial support of this project. We are also grateful to Prof. Concepta M. McManus for English correction.
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do Almo, M.M. et al. (2020). Anti-CD3 Stimulated T Cell Transcriptome Reveals Novel ncRNAs and Correlates with a Suppressive Profile. In: Setubal, J.C., Silva, W.M. (eds) Advances in Bioinformatics and Computational Biology. BSB 2020. Lecture Notes in Computer Science(), vol 12558. Springer, Cham. https://doi.org/10.1007/978-3-030-65775-8_17
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DOI: https://doi.org/10.1007/978-3-030-65775-8_17
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