Pangenomic Identification of Metabolic Switches in Kosakonia cowanii: A Consolidated Network Biology Approach

• Davi Mota Campos UFU
• Gustavo Henrique Leal da Fonseca UFU
• Arthur Rodrigues Cândido UFU
• Nilvanira Donizete Tebaldi UFU
• Lara Caroline Borges Moreira Mota UFU
• Joberth Lee Corrêa UFU
• Ana Carolina Costa Santos UFU
• Carlos Ueira Vieira UFU
• Anderson Rodrigues dos Santos UFU

DOI: https://doi.org/10.5753/eritm.2026.27154

Resumo

Kosakonia cowanii acts as both a plant growth-promoting rhizobacterium (PGPR) and an opportunistic pathogen. To characterize this lifestyle transition, we built a pangenomic pipeline across 77 genomes, consolidating strain-specific interactomes into a species-level graph. Biological coherence filters discarded 12,000 false-positive orthology mappings. Applying Bridging Centrality (BriCe) with functional bridging criteria identified 46 conserved metabolic switches at the PGPR-pathogen interface. These switches, including flagellar regulators and MFS transporters, are tractable targets for experimental validation.

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