Genomic and phylogenetic analysis of plant growth-promoting bacteria

• Marcus Vinicius da Silva Rodrigues UTFPR
• João Paulo de Oliveira
• Sandriele Aparecida Noriler UEL
• Angelo Barreiro Garcia UEL
• Ulisses de Pádua Pereira UEL
• Ulisses Nunes da Rocha Helmholtz Centre for Environmental Research
• Admilton Gonçalves de Oliveira Junior UEL
• Glaucia Maria Bressan UTFPR

DOI: https://doi.org/10.5753/bsb.2024.245593

Abstract

This study aims to analyze the genomic characteristics of a plant growth-promoting bacterium, focusing on genome assembly and annotation, as well as phylogenetic and metabolic analyses. The primary objective is to identify and validate genes related to potassium and phosphate solubilization, as well as other primary and secondary metabolic pathways. The research involves the selection of a strain, cultivation, DNA extraction, sequencing, genome assembly and annotation, followed by phylogenetic and comparative analyses. The analyzed Bacillus nitratireducensLABIM48 strain shows high genomic conservation, indicating a common origin and conserved evolution. Phylogenetic analyses confirmed that the strain belongs to the same mentioned species, with the potential to produce bioactive compounds of interest identified in silico. The ability to solubilize phosphate and potassium was validated through in vitro tests.

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