Genomic and phylogenetic analysis of plant growth-promoting bacteria
Resumo
Este estudo busca analisar as características genômicas de uma bactéria promotora de crescimento de plantas, com foco na montagem e anotação do genoma, além de análises filogenéticas e metabólicas. O objetivo principal é identificar e validar genes relacionados à solubilização de potássio e fosfato, bem como a outras vias metabólicas primárias e secundárias. A pesquisa envolve a seleção de uma linhagem, cultivo, extração de DNA, sequenciamento, montagem e anotação genômica, seguida por análises filogenéticas e comparativas. A linhagem de Bacillus nitratireducensLABIM48 analisada apresenta alta conservação genômica, indicando uma origem comum e evolução conservada. As análises filogenéticas confirmaram que a linhagem pertence à mesma espécie mencionada, com potencial para produzir compostos bioativos de interesse identificados in silico. A capacidade de solubilizar fosfato e potássio foi validada por testes in vitro.Referências
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Christensen, H. and Olsen, J.E. (2022) 'Introduction to phylogenetic analysis of molecular sequence data', Learning Materials in Biosciences, DOI: 10.1007/978-3-031-45293-2_6.
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Debasis, Mitra., Marika, P. and Beatriz, E.G.S. (2024) 'Interaction between plants and growth-promoting rhizobacteria (PGPR) for sustainable development', Bacteria, DOI: 10.3390/bacteria3030009.
Dixit, R., Kapoor, N., Singh, N. and Kumar, A. (2023) 'Bioinformatics resources for sustainable agriculture', DOI: 10.4018/979-8-3693-3026-5.ch015.
Faizan, M., Singh, A., Eren, A., Sultan, H., Sharma, M., Djalović, I. and Trivan, G. (2024) 'Small molecule, big impacts: nano-nutrients for sustainable agriculture and food security', Journal of Plant Physiology, DOI: 10.1016/j.jplph.2024.154305.
Fernando, G.R., Abeysinghe, S., Rajapakse, R.G.S.C. and Gamage, W.S.M.K. (2024) 'Identification of plant growth-promoting rhizobacteria for the development of probiotic consortia', Proceedings of the International Forestry and Environment Symposium, DOI: 10.31357/fesympo.v28.7039.
Jain, P.M., Cheruvari, A., Tamilselvan, H.M.J. and Kammara, R. (2024) 'Bacillus subtilis comparative genomics', DOI: 10.1099/acmi.0.000810.v1.
Kalyanasundaram, G.T., Syed, N. and Subburamu, K. (2020) 'Recent developments in plant growth-promoting rhizobacteria (PGPR) for sustainable agriculture', DOI: 10.1016/B978-0-12-821406-0.00017-5.
Mahawar, S. (2024) 'Bioinoculants: an ecofriendly approach towards artificial fertilizers in sustainable agriculture', DOI: 10.58532/v3bfbt1p1ch21.
Nichols, R.G. and Davenport, E. (2024) 'Clade-specific long-read sequencing increases the accuracy and specificity of the gyrB phylogenetic marker gene', DOI: 10.1101/2024.07.15.603574.
Ramírez-Pool, J.A., Calderón-Pérez, B., Ruiz-Medrano, R., Ortiz-Castro, R. and Xoconostle-Cázares, B. (2024) 'Bacillus strains as effective biocontrol agents against phytopathogenic bacteria and promoters of plant growth', Microbial Ecology, DOI: 10.1007/s00248-024-02384-1
Rufus, A.C., Kalai, K. and Elangovan, E. (2024) 'Isolation and characterization of Bacillus species for the detection of siderophores', Research Journal of Biotechnology, DOI: 10.25303/1908rjbt092099.
Sarvendra, V.S. (2024) 'Bioinformatics – Supporting modern life science research, applications, and challenges', Brazilian Journal of Development, DOI: 10.34117/bjdv10n2-011.
Setiawati, T.C. et al. (2022) 'Use of Bacillus as a plant growth-promoting rhizobacteria to improve phosphate and potassium availability in acidic and saline soils', KnE Life Sciences, pp. 541–558.
Tongrui, Zhang., Qinhao, J., Li, G., Linlin, L., Fei, L. and Can, Z. (2024) 'Plant growth-promoting rhizobacteria (PGPR) improve the growth and quality of several crops', Heliyon, DOI: 10.1016/j.heliyon.2024.e31553.
Yin, Q.J., Ying, T.T., Zhou, Z.Y., Hu, G., Yang, C., Hua, Y., Wang, H. and Wei, B. (2023) 'Species-specificity of the secondary biosynthetic potential in Bacillus', Frontiers in Microbiology, DOI: 10.3389/fmicb.2023.1271418.
Zia, A. (2024) 'Plant growth promoting rhizobacteria: a novel approach towards sustainable agriculture', Deleted Journal, DOI: 10.37446/corbio/ra/2.1.2024.16-28.
Bahadur, I. et al. (2019) 'Potassium-solubilizing bacteria (KSB): a microbial tool for K-solubility, cycling, and availability to plants', in Plant Growth Promoting Rhizobacteria for Agricultural Sustainability: From Theory to Practices, pp. 257-265.
Christensen, H. and Olsen, J.E. (2022) 'Introduction to phylogenetic analysis of molecular sequence data', Learning Materials in Biosciences, DOI: 10.1007/978-3-031-45293-2_6.
Das, V.M., Gautam, B., Yadav, P.K. and Singh, S. (2024) 'Identification of conserved pathways in Bacillus strains known for plant growth-promoting behavior using a multifaceted computational approach', Agriculture, DOI: 10.3390/agriculture14060838.
Debasis, Mitra., Marika, P. and Beatriz, E.G.S. (2024) 'Interaction between plants and growth-promoting rhizobacteria (PGPR) for sustainable development', Bacteria, DOI: 10.3390/bacteria3030009.
Dixit, R., Kapoor, N., Singh, N. and Kumar, A. (2023) 'Bioinformatics resources for sustainable agriculture', DOI: 10.4018/979-8-3693-3026-5.ch015.
Faizan, M., Singh, A., Eren, A., Sultan, H., Sharma, M., Djalović, I. and Trivan, G. (2024) 'Small molecule, big impacts: nano-nutrients for sustainable agriculture and food security', Journal of Plant Physiology, DOI: 10.1016/j.jplph.2024.154305.
Fernando, G.R., Abeysinghe, S., Rajapakse, R.G.S.C. and Gamage, W.S.M.K. (2024) 'Identification of plant growth-promoting rhizobacteria for the development of probiotic consortia', Proceedings of the International Forestry and Environment Symposium, DOI: 10.31357/fesympo.v28.7039.
Jain, P.M., Cheruvari, A., Tamilselvan, H.M.J. and Kammara, R. (2024) 'Bacillus subtilis comparative genomics', DOI: 10.1099/acmi.0.000810.v1.
Kalyanasundaram, G.T., Syed, N. and Subburamu, K. (2020) 'Recent developments in plant growth-promoting rhizobacteria (PGPR) for sustainable agriculture', DOI: 10.1016/B978-0-12-821406-0.00017-5.
Mahawar, S. (2024) 'Bioinoculants: an ecofriendly approach towards artificial fertilizers in sustainable agriculture', DOI: 10.58532/v3bfbt1p1ch21.
Nichols, R.G. and Davenport, E. (2024) 'Clade-specific long-read sequencing increases the accuracy and specificity of the gyrB phylogenetic marker gene', DOI: 10.1101/2024.07.15.603574.
Ramírez-Pool, J.A., Calderón-Pérez, B., Ruiz-Medrano, R., Ortiz-Castro, R. and Xoconostle-Cázares, B. (2024) 'Bacillus strains as effective biocontrol agents against phytopathogenic bacteria and promoters of plant growth', Microbial Ecology, DOI: 10.1007/s00248-024-02384-1
Rufus, A.C., Kalai, K. and Elangovan, E. (2024) 'Isolation and characterization of Bacillus species for the detection of siderophores', Research Journal of Biotechnology, DOI: 10.25303/1908rjbt092099.
Sarvendra, V.S. (2024) 'Bioinformatics – Supporting modern life science research, applications, and challenges', Brazilian Journal of Development, DOI: 10.34117/bjdv10n2-011.
Setiawati, T.C. et al. (2022) 'Use of Bacillus as a plant growth-promoting rhizobacteria to improve phosphate and potassium availability in acidic and saline soils', KnE Life Sciences, pp. 541–558.
Tongrui, Zhang., Qinhao, J., Li, G., Linlin, L., Fei, L. and Can, Z. (2024) 'Plant growth-promoting rhizobacteria (PGPR) improve the growth and quality of several crops', Heliyon, DOI: 10.1016/j.heliyon.2024.e31553.
Yin, Q.J., Ying, T.T., Zhou, Z.Y., Hu, G., Yang, C., Hua, Y., Wang, H. and Wei, B. (2023) 'Species-specificity of the secondary biosynthetic potential in Bacillus', Frontiers in Microbiology, DOI: 10.3389/fmicb.2023.1271418.
Zia, A. (2024) 'Plant growth promoting rhizobacteria: a novel approach towards sustainable agriculture', Deleted Journal, DOI: 10.37446/corbio/ra/2.1.2024.16-28.
Publicado
02/12/2024
Como Citar
RODRIGUES, Marcus Vinicius da Silva; OLIVEIRA, João Paulo de; NORILER, Sandriele Aparecida; GARCIA, Angelo Barreiro; PEREIRA, Ulisses de Pádua; ROCHA, Ulisses Nunes da; OLIVEIRA JUNIOR, Admilton Gonçalves de; BRESSAN, Glaucia Maria.
Genomic and phylogenetic analysis of plant growth-promoting bacteria. In: SIMPÓSIO BRASILEIRO DE BIOINFORMÁTICA (BSB), 17. , 2024, Vitória/ES.
Anais [...].
Porto Alegre: Sociedade Brasileira de Computação,
2024
.
p. 131-142.
ISSN 2316-1248.
DOI: https://doi.org/10.5753/bsb.2024.245593.
