Image-based automatic counting of spotted fever-carrying tick larvae in vitro
Resumo
Spotted fever or tick fever, a disease transmitted by ticks, has a high case-fatality rate when a treatment is not started early. To reduce the cases of tick fever, many acaricides are used for keeping control of tick population. However, these tests are done manually. To choose the most effective acaricide, a specialist has to count how many ticks are dead and how many are still alive after subjecting them to it. This test is time-consuming, so this work aims to automate this procedure. Therefore, it is proposed a computational methodology to automate this procedure through image processing. Through the proposed method, 99,14% of ticks larvae were found, and 99.34% of alive and 96.89% of dead ticks larvae were counted correctly in this work.
Referências
R. P. de Araújo, M. B. M. de Albuquerque Navarro, and T. A. de Oliveira Cardoso, Febre maculosa no brasil: estudo da mortalidade para a vigilância epidemiológica. Cadernos Saúde Coletiva, vol. 24, no. 3, 2016.
-- G1, Em 18 meses, pás registra 250 casos de febre maculosa com 81 mortes, 2018, acessado em 20 de janeiro de [Online]. Available: https://g1.globo.com/jornal-nacional/noticia/2018/08/02/em-18 -meses-pais-registra-250-casos-de-febre-maculosa-com-81-mortes.ghtml
P. D. Cortivo, E. Dias, J. O. J. Barcellos, V. Peripolli, J. B. G. Costa Jr, B. S. L. Dallago, and C. M. McManus, Use of thermographic images to detect external parasite load in cattle, Computers and Electronics in Agriculture, vol. 127, pp. 413 - 417, 2016.
G. Loy and A. Zelinsky, A fast radial symmetry transform for detecting points of interest, in European Conference on Computer Vision. Springer, 2002, pp. 358 - 368. DOI: 10.1007/3-540-47969-4_24
S. Aghayee, D. E. Winkowski, Z. Bowen, E. E. Marshall, M. J. Harrington, P. O. Kanold, and W. Losert, Particle tracking facilitates real time capable motion correction in 2d or 3d two-photon imaging of neuronal activity, Frontiers in neural circuits, vol. 11, p. 56, 2017.
K.-N. Chen, C.-H. Chen, and C.-C. Chang, Efficient illumination compensation techniques for text images, Digital Signal Processing, vol. 22, no. 5, pp. 726 - 733, 2012. DOI: 10.1016/j.dsp.2012.04.010
N. Otsu, A threshold selection method from gray-level histograms, IEEE transactions on systems, man, and cybernetics, vol. 9, no. 1, pp. 62 - 66, 1979.