Anti-CD3 Stimulated T Cell Transcriptome Reveals Novel ncRNAs and Correlates with a Suppressive Profile

• Manuela M. do Almo UnB
• Isabel G. Sousa UnB http://orcid.org/0000-0002-1916-0106
• Waldeyr Mendes Cordeiro da Silva UnB / IFG http://orcid.org/0000-0002-8660-6331
• Thomas Gatter University of Leipzig http://orcid.org/0000-0001-5567-3016
• Peter F. Stadler University of Leipzig / University of Vienna / Universidad Nacional de Colombia / Max Planck Institute for Mathematics in the Sciences / Santa Fe Institute http://orcid.org/0000-0002-5016-5191
• Steve Hoffmann University of Leipzig / Fritz Lipmann Institute / Friedrich-Schiller-University Jena
• Andrea Q. Maranhão UnB / iii-INCT http://orcid.org/0000-0002-1946-7191
• Marcelo Brigido UnB / iii-INCT http://orcid.org/0000-0002-7136-7059

Resumo

T lymphocytes are key players in immunity. Anti-CD3 antibodies activate T cells and promote a suppressive phenotype in vivo. Although the pharmacological use of these antibodies is widely studied, the underlying mechanisms are still poorly understood. Here we describe the response of the non-coding RNA transcriptome of T cells after in vitro stimulation of peripheral blood mononuclear cells (PBMC) by anti-CD3 and demonstrate that several novel long non-coding RNA are associated with antibody treatment. Regulated long intergenic non-coding RNAs were associated with lymphocyte activation and signaling pathways. In particular the lncRNA transcripts WFDC21P and GAPLINC are regulated in stimulated T cell.

Palavras-chave: Anti-CD3, lncRNA, Gene regulation, Alternative splicing, Transcriptome